GFP antibody [GT7312]
Catalogue Number: GTX628894-GTX
GTX628894 Image
GTX628894 Image
GTX628894 Image
GTX628894 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (10 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody [GT7312] (GTX628894) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX628894 Image
ELISA detection of GFP using GTX628894 for capture and GTX113617 for detection. Coating Ab: mouse anti-GFP (GTX628894, 2µg/well ) Sample: GFP protein Detected Ab: rabbit anti-GFP (GTX113617) Conjugated Ab: goat anti-rabbit IgG-HRP (GTX213110-01, 1:20000)
GTX628894 Image
GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 µg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 µg lysate/extract of transfected 293T cell B. Control with 2 µg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 µg of GFP tag antibody (GTX628894) 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody (GTX628894) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 29 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT7312 |
| Isotype: | IgG2a |
| Immunogen: | Full length GFP recombinant protein |
| Application: | ELISA, IP, sELISA, WB |
Additional Text
Purification
Protein G purified
Concentration
1.04 mg/ml
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
29
Application Notes
WB: 1:5000-1:20000. IP: 1:100-1:500. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX628894 Datasheet
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