CD44 antibody [GT462]
Catalogue Number: GTX628895-GTX
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GTX628895 Image
GTX628895 Image
GTX628895 Image
GTX628895 Image
GTX628895 Image
GTX628895 Image
GTX628895 Image
Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:500.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000.
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Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX628895 Image
Untreated (-) and treated (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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CD44 antibody [GT462] detects CD44 protein at cell membrane by immunohistochemical analysis (Autostainer Formulated). Sample: Paraffin-embedded human breast cancer. CD44 stained by CD44 antibody [GT462] (GTX628895) diluted at 1:200. Antigen Retrieval: EDTA buffer, 20 min
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CD44 antibody detects CD44 protein by flow cytometry analysis. Sample: HL-60 cell fixed in 4% paraformaldehyde at 4oC for 5 min. Black: Isotype control dilution: 1:100. Green: CD44 antibody (GTX628895) dilution: 1:100.
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CD44 antibody [GT462] detects CD44 protein at membrane on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. CD44 antibody [GT462] (GTX628895) dilution: 1:200. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 82 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT462 |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein encompassing a sequence within the Extracellular domain of human CD44. The exact sequence is proprietary. |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq]
Additional Text
Gene Name
CD44
Gene ID
960
Uniprot ID
P16070
Concentration
0.7 mg/ml
Purification
Protein G purified
Molecular Weight
82
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500-1:3000. IHC-P: 1:100-1:1000. FCM: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX628895 Datasheet
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