COX4 antibody [GT854]
Catalogue Number: GTX628901-GTX
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GTX628901 Image
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. COX4 stained by COX4 antibody [GT854] (GTX628901) diluted at 1:200. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
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COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. COX4 stained by COX4 antibody [GT854] (GTX628901) diluted at 1:200. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
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Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX628901 Image
Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: COX4 stained by COX4 antibody (GTX628901) diluted at 1:1000.
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COX4 antibody detects COX4I1 protein by western blot analysis. A. 50 µg mouse heart lysate/extract 12% SDS-PAGE COX4 antibody (GTX628901) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX628901 Image
COX4 antibody [GT854] detects COX4I1 protein by western blot analysis. A. 50 µg rat muscle lysate/extract 15% SDS-PAGE COX4 antibody [GT854] (GTX628901) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 20 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT854 |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]
Additional Text
Gene Name
COX4I1
Molecular Weight
20
Gene ID
1327
Uniprot ID
P13073
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX628901 Datasheet
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