6X His tag antibody [GT359] (HRP)
Catalogue Number: GTX628914-01-GTX
GTX628914-01 Image
GTX628914-01 Image
GTX628914-01 Image
GTX628914-01 Image
GTX628914-01 Image
6X His tag antibody [GT359] (HRP) detects 6xHis Tag protein by Western blot analysis. A. 5 µl non-induced cell lysate from E. coli expressing His-tagged proteins B. 5 µl IPTG-induced cell lysate from E. coli expressing His-tagged proteins 15 % SDS-PAGE 6X His tag antibody [GT359] (HRP) (GTX628914-01) dilution: 1:5000
GTX628914-01 Image
6x His antibody conjugated HRP detects 6x His protein by western blot analysis. IPTG-induced (+, ) cell lysate from E. coli expressing different GST-tagged proteins and His-tagged proteins (2 µl) were separated by 12% SDS-PAGE, and the membrane was blotted with 6x His antibody conjugated HRP (GTX628914-01) at a dilution of 1:20000.
GTX628914-01 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with 6X His tag antibody [GT359] (GTX628914-01) diluted at 1:5000 and competitor's antibody (sc-8036) diluted at 1:5000. *The competitor is not affiliated with GeneTex and does not endorse this product.
GTX628914-01 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [GT359] (GTX628914-01) diluted at 1:5000.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | 6X His tag antibody , His antibody , 6x His antibody , 6x his tag antibody , His Tag antibody , 6xHis Tag antibody , 6 x His antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT359 |
| Isotype: | IgG3 |
| Immunogen: | The immunogen used to generate this antibody corresponds to 6X His tag |
| Application: | ELISA, WB |
Additional Text
Purification
Protein A purified
Concentration
0.14 mg/ml
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX628914-01 Datasheet
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