5-Methylcytosine / 5-mC antibody [GT4111]
Catalogue Number: GTX629448-GTX
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5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleolus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) diluted at 1:200.
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GTX629448 5-methylcytosine antibody [GT4111] Dot blot analysis of anti-5-mC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody (GTX629448) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine
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5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis. Sample: 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.
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5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis. Sample: 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.
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GTX629448 5-Methylcytosine/ 5-mC antibody [GT4111] in MeDIP experiment. Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody [GT4111] (GTX629448) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.
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5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT4111 |
| Isotype: | IgG1 |
| Immunogen: | The immunogen used to generate this antibody corresponds to 5-Methylcytosine / 5-mC |
| Application: | IHC-P, EM, IHC-Fr, DB, IHC-WM, MeDIP |
Additional Text
Purification
Protein G purified
Concentration
1.7 mg/ml
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
IHC-P: 1:100-1:1000. IHC-Wm: 1:50-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
5-mC antibody detects methylated cytosine, which is a key epigenetic regulatory modification of genomic DNA and is considered a "fifth base". The 5-mC mark is added by DNA methyltransferases (DNMTs) and serves predominately to turn off gene transcription. It is reversible via a DNA demethylation pathway triggered by the Ten-eleven translocation (TET) family of dioxygenases.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX629448 Datasheet
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