p21 Cip1 antibody [GT1032]
Catalogue Number: GTX629543-GTX
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p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody (GTX102993) diluted at 1:1000. Red: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500. Blue: Hoechst 33342 staining.
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p21 Cip1 antibody [GT1032] immunoprecipitates CDKN1A protein in IP experiments. IP samples: HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr A. 30 µg HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr B. Control with 4 µg of preimmune Mouse IgG C. Immunoprecipitation of CDKN1A protein by 4 µg p21 Cip1 antibody [GT1032] (GTX629543) 15 % SDS-PAGE The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:1000. [EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
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p21 Cip1 antibody [GT1032] (GTX629543) detects p21 Cip1 by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: p21 Cip1 antibody [GT1032] (GTX629543) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
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p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 µm.
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p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. p21 Cip1 stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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The data was published in the journal PLoS One in 2015. PMID: 25961745
GTX629543 Image
The data was published in the journal PLoS One in 2015. PMID: 25961745
GTX629543 Image
The data was published in the journal PLoS One in 2015. PMID: 25961745
GTX629543 Image
Untreated (-) and treated (+) HCT-116 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX629543 Image
Untreated (-) and treated (+) HCT 116 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 18 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | cyclin dependent kinase inhibitor 1A , CAP20 , CDKN1 , CIP1 , MDA-6 , P21 , SDI1 , WAF1 , p21CIP1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT1032 |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary. |
| Application: | FC, ICC, IF, IHC-P, IP, WB |
Description
Description: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]
Additional Text
Gene ID
1026
Gene Name
CDKN1A
Uniprot ID
P38936
Concentration
0.8 mg/ml
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
p21 (Cip1) antibody recognizes p21Cip1 protein, a cyclin-dependent kinase inhibitor with a predicted molecular weight of 18 kDa. p21 is a major downstream regulator of p53 activity in response to DNA damage to control cell cycle arrest. p21 also interacts with proliferating cell nuclear antigen (PCNA) to inhibit PCNA-dependent S-phase DNA synthesis.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX629543 Datasheet
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