beta Actin antibody [GT5512]
Catalogue Number: GTX629630-GTX
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beta Actin antibody [GT5512] detects beta Actin protein at cell membrane and cytoplasm in rat adrenal gland by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. beta Actin antibody [GT5512] (GTX629630) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg drosophila lysate/extract 10% SDS-PAGE Beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg goat blood lysate/extract 10% SDS-PAGE beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg 293T whole cell lysate/extract D. 30 µg A431 whole cell lysate/extract E. 30 µg HeLa whole cell lysate/extract F. 30 µg HepG2 whole cell lysate/extract G. 30 µg H1299 whole cell lysate/extract H. 30 µg HCT116 whole cell lysate/extract I. 30 µg MCF-7 whole cell lysate/extract J. 30 µg NT2D1 whole cell lysate/extract K. 30 µg PC-3 whole cell lysate/extract L. 30 µg U87-MG whole cell lysate/extract 10% SDS-PAGE Beta Actin antibody [GT5512] (GTX629630) dilution: 1:20000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg mouse brain lysate/extract D. 30 µg PC-12 whole cell lysate/extract E. 30 µg rat brain lysate/extract 10% SDS-PAGE Beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg rabbit blood lysate/extract 10% SDS-PAGE beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 20 µg 293T whole cell lysate/extract B. 10 µg 293T whole cell lysate/extract C. 5 µg 293T whole cell lysate/extract D. 1 µg 293T whole cell lysate/extract 10% SDS-PAGE Beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 10 µg yeast lysate/extract 10% SDS-PAGE beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg 30 hpf zebrafish lysate/extract 10% SDS-PAGE beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [GT5512] (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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beta Actin antibody [GT5512] detects beta Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog muscle. beta Actin stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: PG-4 cells were fixed in ice-cold MeOH for 5 min. Green: beta Actin stained by beta Actin antibody? (GTX629630) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 µm.
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beta Actin antibody[GT5512] detects beta Actin protein by western blot analysis. A. 30 µg MCF-7 whole cell lysate/extract B. 30 µg MDA-MB-231 whole cell lysate/extract 10% SDS-PAGE beta Actin antibody[GT5512] (GTX629630) dilution: 1:20000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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beta Actin antibody [GT5512] detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min. Green: beta Actin protein stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500. Blue: Hoechst 33342 staining.
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The data was published in the 2021 in Transl Neurosci. PMID: 33708438
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The data was published in the 2021 in Transl Neurosci. PMID: 33708438
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The data was published in the journal Front Cell Neurosci in 2018. PMID: 30337858
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The data was published in the journal PLoS One in 2014. PMID: 25141004
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The data was published in the journal PLoS One in 2017. PMID: 28957379
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The data was published in the journal Cell Death Dis in 2013. PMID: 24008737
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The data was published in the journal Front Immunol in 2019. PMID: 31787977
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The data was published in the journal Cell Discov in 2018. PMID: 30455981
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The data was published in the journal Toxins (Basel) in 2016. PMID: 27827950
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The data was published in the journal Toxins (Basel) in 2016. PMID: 27827950
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The data was published in the journal Sci Rep in 2017. PMID: 28169314
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The data was published in the journal Oncotarget in 2017.PMID: 28537905
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The data was published in the journal Front Immunol in 2017.PMID: 29021793
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The data was published in the journal Sci Rep in 2019.PMID: 30816208
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The data was published in the journal Sci Rep in 2019.PMID: 30816208
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The data was published in the journal Genes (Basel) in 2020.PMID: 32085655
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The data was published in the journal Neuron in 2018.PMID: 29861284
GTX629630 Image
The data was published in the journal Sci Rep in 2019.PMID: 30816208
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Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no Preservative|No Preservative |
| Molecular Weight: | 42 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | actin beta , BRWS1 , PS1TP5BP1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT5512 |
| Isotype: | IgG1 |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]
Additional Text
Gene Name
ACTB
Purification
Protein G purified
Gene ID
60
Uniprot ID
P60709
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX629630 Datasheet
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