5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]
Catalogue Number: GTX629765-GTX
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GTX629765 5-hydroxymethylcytosine antibody [GT13612] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629765) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine
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5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] (GTX629765) dilution: 1:100. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] (GTX629765) dilution: 1:100. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX629765 Image
5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] (GTX629765) dilution: 1:100. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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GTX629765 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] in MeDIP experiment. Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] (GTX629765) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | 5hmc Antibody , 5-hmc Antibody , 5-HydroxymethylCytidine Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT13612 |
| Isotype: | IgG2b |
| Immunogen: | The immunogen used to generate this antibody corresponds to 5-HydroxymethylCytosine / 5-hmC |
| Application: | ICC, IF, IHC-P, EM, IHC-Fr, DB, MeDIP |
Additional Text
Concentration
0.81 mg/ml
Purification
Affinity Purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
5-hmC antibody recognizes 5-Hydroxymethylcytosine (5-hmC), which is referred to as a "sixth base" of mammalian genomic DNA. 5-hmC is thought to be an epigenetic regulatory modification in its own right, while also being the product of the first TET protein-catalyzed oxidation of 5-methylcytosine in the DNA demethylation pathway.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX629765 Datasheet
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