ROCK1 + ROCK2 antibody [GT261]
Catalogue Number: GTX629971-GTX
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Untreated (-) and treated (+) PC-12 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [GT261] (GTX629971) diluted at 1:500.
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Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [GT261] (GTX629971) diluted at 1:2000.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 (GTX629971) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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ROCK1 + ROCK2 antibody [GT261] detects ROCK1 + ROCK2 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg BCL-1 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 5 % SDS-PAGE ROCK1 antibody [GT261] (GTX629971) dilution: 1:1000
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ROCK1 + ROCK2 antibody [GT261] detects ROCK1 + ROCK2 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 + ROCK2 antibody [GT261] (GTX629971) dilution: 1:1000
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ROCK1 + ROCK2 antibody [GT261] detects ROCK1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 + ROCK2 antibody [GT261] (GTX629971) dilution: 1:1000
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 158 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT261 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary. |
| Application: | WB |
Description
Description: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]
Additional Text
Purification
Protein G purified
Molecular Weight
158
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Gene ID
6093; 9475
Gene Name
ROCK1, ROCK2
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Uniprot ID
O75116, Q13464
GTX629971 Datasheet
SUPPORT
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DELIVERY
we offer free delivery to UK universities and non profit organisations