Calnexin antibody [GT1563]
Catalogue Number: GTX629976-GTX
GTX629976 Image
GTX629976 Image
GTX629976 Image
GTX629976 Image
GTX629976 Image
GTX629976 Image
GTX629976 Image
GTX629976 Image
GTX629976 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:6000.
GTX629976 Image
Calnexin antibody [GT1563] detects Calnexin protein by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Calnexin stained by Calnexin antibody [GT1563] (GTX629976) diluted at 1:500. Red: Calnexin, stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500. Blue: Hoechst 33342 staining.
GTX629976 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX629976 Image
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX629976 Image
Calnexin antibody [GT1563] detects Calnexin protein at cytoplasm by immunofluorescent analysis. Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calnexin stained by Calnexin antibody [GT1563] (GTX629976) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX629976 Image
Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 7.5 % SDS-PAGE Calnexin antibody [GT1563] (GTX629976) dilution: 1:1000
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Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [GT1563] (GTX629976) dilution: 1:500
GTX629976 Image
Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [GT1563] (GTX629976) dilution: 1:1000
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 68 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | calnexin , CNX , IP90 , P90 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT1563 |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary. |
| Application: | ICC, IF, IP, WB, DB |
Description
Description: This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]
Additional Text
Gene ID
821
Gene Name
CANX
Uniprot ID
P27824
Purification
Protein G purified
Molecular Weight
68
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX629976 Datasheet
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