TDP43 antibody [GT225]
Catalogue Number: GTX630196-GTX
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GTX630196 Image
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT225] (GTX630196) diluted at 1:500.
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TDP43 antibody [GT225] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. TDP43 antibody [GT225] (GTX630196) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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TDP43 antibody [GT225] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TDP43 antibody [GT225] (GTX630196) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Wild-type (WT) and TARDBP knockout (KO) HAP1 cell extracts (50 Aµg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Data provided byA?YCharOSA?Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
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TARDBP antibody [GT225] detects TARDBP protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract B. 30 µg Raw264.7 whole cell lysate/extract 10 % SDS-PAGE TARDBP antibody [GT225] (GTX630196) dilution: 1:1000
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TARDBP antibody [GT225] detects TARDBP protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 10 % SDS-PAGE TARDBP antibody [GT225] (GTX630196) dilution: 1:1000
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TARDBP antibody [GT225] validation by siRNA knock-down. Upperpanel: TARDBP antibody [GT225] GTX630196 Lower panel: GAPDH antibody (GTX100118) A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells 10 % SDS-PAGE TARDBP antibody [GT225] (GTX630196) dilution: 1:3000 GAPDH antibody (GTX100118) dilution: 1:10000
GTX630196 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no Preservative|No Preservative |
| Molecular Weight: | 45 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | TAR DNA binding protein , ALS10 , TDP-43 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT225 |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary. |
| Application: | IHC-P, WB |
Description
Description: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]
Additional Text
Gene Name
TARDBP
Purification
Protein G purified
Gene ID
23435
UniProt ID
Q13148
Molecular Weight
45
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX630196 Datasheet
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