Histone H4K20me2 (dimethyl Lys20) antibody [GT282]
Catalogue Number: GTX630544-GTX
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Immunoprecipitation of HIST1H4A protein from HeLa whole cell extracts using 5 µg of Histone H4K20me2 (dimethyl Lys20) antibody [GT282] (GTX630544). Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody [GT282] (GTX630544). EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
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Dot blot analysis of various of H4K20 methylation peptides using Histone H4K20me2 (dimethyl Lys20) antibody [GT282] (GTX630544) at antibody dilution of 1:500. Un : H4 protein peptide Me : H4K20 mono-methyl?peptide 2Me : H4K20 di-methyl?peptide 3Me : H4K20 Tri-methyl?peptide
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Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.
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Histone H4 (di-Methyl Lys20) antibody [GT282] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT282] (GTX630544) diluted at 1:500. Blue: Hoechst 33342 staining.
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Histone H4 (di methyl Lys20) antibody [GT282] detects Histone H4 (di methyl Lys20) protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [GT282] (GTX630544) dilution: 1:5000
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The data was published in the journal F1000Res in 2018. PMID: 30498568
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The data was published in the journal F1000Res in 2018. PMID: 30498568
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT282 |
| Isotype: | IgG2b |
| Immunogen: | Carrier-protein conjugated synthetic peptide surrounding di-methyl Lys20 of human Histone H4. The exact sequence is proprietary. |
| Application: | ICC, IF, IP, ChIP, WB, DB |
Additional Text
Purification
Protein G purified
Gene ID
8359
Modification
Dimethylated, Lys20
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Gene Name
H4C1
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Dot: 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX630544 Datasheet
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