SA1 antibody [GT1515]
Catalogue Number: GTX632010-GTX
GTX632010 Image
GTX632010 Image
GTX632010 Image
GTX632010 Image
GTX632010 Image
GTX632010 Image
SA1 antibody [GT1515] detects SA1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SA1 protein stained by SA1 antibody [GT1515] (GTX632010) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
GTX632010 Image
Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 µg of SA1 antibody [GT1515] (GTX632010). Western blot analysis was performed using SA1 antibody [GT1515] (GTX632010). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
GTX632010 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (GTX632010) diluted at 1:500.
GTX632010 Image
Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (GTX632010) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX632010 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (GTX632010) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 144 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | stromal antigen 1 , MRD47 , SA1 , SCC3A |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT1515 |
| Isotype: | IgG2b |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SA1. The exact sequence is proprietary. |
| Application: | ICC, IF, IP, WB |
Description
Description: This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq]
Additional Text
Gene Name
STAG1
Uniprot ID
Q8WVM7
Concentration
0.9 mg/ml
Gene ID
10274
Molecular Weight
144
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX632010 Datasheet
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