SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9]

Catalogue Number: GTX632604-GTX

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FACS analysis of 293T cells infected with recombinant vaccinia virus carrying the Spike gene using GTX632604 SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9].

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SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 by immunofluorescent analysis. Sample: Vero E6 cells were infected with SARS-CoV-2 (MOI of 1) and fixed with chilled methanol/acetone. Green: SARS-CoV-2 infected Vero E6 cells stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604). Blue: Nuclei were counterstained with DAPI. *From Zheng Z, et al. bioRxiv(2020). Shown under license agreement

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SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis of infected human placenta.

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SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis of SARS-CoV-2 infected human lung. The IHC-P was performed by HISTOWIZ.

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Non-infected (-) and infected (+, 48h pl MOI 0.01) Caco-2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.

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SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis. Sample: BHK-21 cells transfected with full-length SARS-CoV-2 spike were fixed in 4% paraformaldehyde at RT for 30 min. Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:2000.

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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Immunoprecipitation of SARS-CoV-2 Spike transfected 293T whole cell extracts using 2 µg of SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604). Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604). EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.

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Indirect ELISA analysis was performed by coating plate with 50 µL of recombinant SARS-CoV-2 (COVID-19) spike S2 ECD protein at concentrations ranging from 0.0625 µg/mL to 4 µg/mL. The coated protein is detected with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) at 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

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SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (GTX136059-pro, 0.25 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (GTX136061-pro, 0.2 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 µg/mL and GTX135360 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 µg/mL and GTX135356 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using GTX632604 as capture antibody at concentration of 5 µg/mL and GTX635672 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 µg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV / SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis. Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block. Red: SARS-CoV / SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000. Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640). Red: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag protein (GTX135684-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 µg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 µg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green:SARS-CoV-2 (COVID-19) spike protein stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).

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Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below. Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 µg/mL) Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 µg/mL)

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Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 µg/mL and Human IgG antibody (HRP) (1:200000) as detection antibody.

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Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (501.V1) spike protein, SARS-CoV-2 (501.V2) spike protein and SARS-CoV-2 spike protein (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 µg/mL). Mouse IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

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Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 µg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro), SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro), and SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 µg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro) and SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 µg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1039] (GTX635911) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 µg/mL). Mouse IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

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Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 µg/mL). Mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

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Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below. Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 µg/mL) Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 µg/mL).

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Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using antibodies as below. Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 µg/mL) Detection: Human IgG antibody (HRP) (1:200000 )

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Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / Omicron variant, His tag (GTX136780-pro) (29.71-0.46 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 µg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

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The data was published in the 2022 in Int J Mol Sci. PMID: 35805887

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The data was published in the 2022 in Int J Mol Sci. PMID: 35805887

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The data was published in the 2020 in Sci Rep. PMID: 33318562

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Manufacturer:	GeneTex
Preservative:	no preservative\|No Preservative
Physical state:	Liquid
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 25 ul
Host name:	Mouse
Clone:	1A9
Isotype:	IgG1
Immunogen:	The immunogen used to generate this antibody corresponds to SARS-CoV SΔ10 (within S2 domain) protein (1029-1192 a.a.). (SARS-CoV strain: Sin2774) The exact sequence is proprietary.
Application:	ELISA, FC, ICC, IF, IHC-P, IP, sELISA, EM, WB, IHC-Fr, IHC

Overview
Datasheets

Additional Text

Purification

Protein G purified

Antibody Clonality

Monoclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Concentration

1 mg/ml

Application Notes

WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. IHC-P: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

GTX632604 Datasheet