Heme Oxygenase 1 antibody [GT17811]
Catalogue Number: GTX633677-GTX
GTX633677 Image
GTX633677 Image
GTX633677 Image
GTX633677 Image
GTX633677 Image
GTX633677 Image
Untreated (-) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:1000.
GTX633677 Image
Heme Oxygenase 1 antibody [GT17811] detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar= 10µm.
GTX633677 Image
Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using GTX633677 as capture antibody at concentration of 5 µg/mL and GTX101147 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
GTX633677 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX633677 Image
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 33 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT17811 |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, sELISA, WB |
Description
Description: Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]
Additional Text
Gene Name
HMOX1
Uniprot ID
P09601
Gene ID
3162
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
33
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX633677 Datasheet
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