Enterovirus D68 VP1 antibody [GT11610]
Catalogue Number: GTX633688-GTX
GTX633688 Image
GTX633688 Image
GTX633688 Image
GTX633688 Image
GTX633688 Image
GTX633688 Image
GTX633688 Image
Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below. Capture: Enterovirus D68 VP1 antibody [GT11610] (GTX633688) (5 µg/mL) Detection: Enterovirus D68 VP1 antibody [HL1997] (GTX637898) (1 µg/mL)
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Enterovirus D68 VP1 antibody [GT11610] detects Enterovirus D68 VP1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mock and Enterovirus D68 VP1 transfected 293T cell. Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT11610] (GTX633688) diluted at 1:4000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Non-infected (-) and infected (+) RD whole cell extracts (5 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT11610] (GTX633688) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus D68 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus D68 VP1 antibody [GT11610] (GTX633688) (1 µg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.
GTX633688 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT11610] (GTX633688) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX633688 Image
Enterovirus D68 VP1 antibody [GT11610] detects Enterovirus D68 VP1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT11610] (GTX633688) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
| Manufacturer: | GeneTex |
| Preservative: | no Preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Enterovirus D68 Capsid protein VP1 , Enterovirus D68 VP1 , EVD68 Capsid protein VP1 , EVD68 VP1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT11610 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of Enterovirus D68 VP1 protein. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, sELISA, WB, IHC |
Additional Text
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. Sandwich ELISA: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX633688 Datasheet
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