VE-Cadherin antibody [GT1369]
Catalogue Number: GTX633705-GTX
GTX633705 Image
GTX633705 Image
GTX633705 Image
GTX633705 Image
VE-cadherin antibody [GT1369] detects VE-cadherin protein at cell membrane by immunofluorescent analysis. Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VE-cadherin protein stained by VE-cadherin antibody [GT1369] (GTX633705) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
GTX633705 Image
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VE-Cadherin antibody [GT1369] (GTX633705) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX633705 Image
VE-Cadherin antibody [GT1369] detects VE-Cadherin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. VE-Cadherin stained by VE-Cadherin antibody [GT1369] (GTX633705) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | no Preservative|No Preservative |
| Molecular Weight: | 88 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | cadherin 5 , 7B4 , CD144 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT1369 |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide encompassing a sequence within the Extracellular domain of human VE-cadherin. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq]
Additional Text
Gene Name
CDH5
Uniprot ID
P33151
Gene ID
1003
Purification
Protein G purified
Molecular Weight
88
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX633705 Datasheet
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