NF-H antibody [GT114]
Catalogue Number: GTX634289-GTX
GTX634289 Image
GTX634289 Image
GTX634289 Image
GTX634289 Image
GTX634289 Image
GTX634289 Image
GTX634289 Image
GTX634289 Image
GTX634289 Image
NF-H antibody [GT114] detects NF-H protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: NF-H protein stained by NF-H antibody [GT114] (GTX634289) diluted at 1:250. Red: NeuN, stained by NeuN antibody (GTX133127) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX634289 Image
NF-H antibody [GT114] detects NF-H protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: NF-H protein stained by NF-H antibody [GT114] (GTX634289) diluted at 1:250. Red: NeuN, stained by NeuN antibody (GTX133127) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX634289 Image
NF-H antibody [GT114] detects NF-H protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NF-H antibody [GT114] (GTX634289) diluted at 1:500. Scale bar = 1 µm. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX634289 Image
NF-H antibody [GT114] detects NF-H protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NF-H antibody [GT114] (GTX634289) diluted at 1:500. Scale bar = 1 µm. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX634289 Image
NF-H antibody [GT114] detects NF-H protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NF-H protein stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
GTX634289 Image
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NF-H antibody (GTX634289) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX634289 Image
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with NF-H antibody [GT114] (GTX634289) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX634289 Image
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with NF-H antibody [GT114] (GTX634289) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 112 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | neurofilament heavy , CMT2CC , NFH |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT114 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human NF-H. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq]
Additional Text
Gene Name
NEFH
Gene ID
4744
Purification
Protein G purified
Uniprot ID
P12036
Molecular Weight
112
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX634289 Datasheet
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