RAS (G12D Mutant) antibody [HL10]
Catalogue Number: GTX635362-GTX
GTX635362 Image
GTX635362 Image
GTX635362 Image
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GTX635362 Image
GTX635362 Image
GTX635362 Image
RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein by immunohistochemical analysis. Sample: Paraffin-embedded human wild-type (left) and KRAS G12D mutant (right) pancreatic tumor. RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:50.
GTX635362 Image
Various whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:1000.
GTX635362 Image
RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein at by immunohistochemical analysis. Sample: Paraffin-embedded KPC mouse pancreatic tumor cells implanted in the pancreas of B6 mice (left) and normal mouse pancreas (right). RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:75. Antigen Retrieval: Citrate buffer, pH 6.0, 40 min
GTX635362 Image
Non-transfected (-) and transfected (+) Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635362 Image
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL10 |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide surrounding mutant G12D of human K-Ras. The exact sequence is proprietary. |
| Application: | IHC-P, WB |
Additional Text
Concentration
1.5 mg/ml
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. IHC-P: 1:25-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
KRAS has the distinction of being a preeminent oncoprotein as it is mutated in more than 85% of RAS-altered cancers. The RAS (G12D Mutant) antibody [HL10] is the first commercially available recombinant antibody that demonstrates exceptional specificity by paraffin-embedded immunohistochemistry and western blot for the RAS G12D mutant protein.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX635362 Datasheet
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