CA19-9 antibody [GT933]
Catalogue Number: GTX635389-GTX
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GTX635389 Image
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CA19-9 antibody [GT933] detects CA19-9 protein at cell membrane by immunofluorescent analysis. Sample: SW1116 (high expression) and HT29 (low expression) cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CA19-9 stained by CA19-9 antibody [GT933] (GTX635389) diluted at 1:1000 and competitor's antibody (MA5-12421 and M66107M) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
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CA19-9 antibody [GT933] (GTX635389) detects CA19-9 by flow cytometry analysis. Sample: COLO 205 cell. Black: Unlabelled sample was used as a control. Red: CA19-9 antibody [GT933] (GTX635389) dilution: 1:50. Blue: Highly cited antibody [116-NS-19-9] dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
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CA19-9 antibody [GT933] specifically detects sialyl-LewisA (Neu5Ac-; CA19-9) on a glycan array. Green: sialyl-LewisA (Neu5Ac-; CA19-9). Red: Modified sialyl-LewisA (Neu5Gc-). Yellow: Positive control. Blue: Negative control.
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Competition analysis of CA 19-9 antibody [GT933] (GTX635389) (330 ng/mL) binding to immobilized recombinant HSA-CA 19-9 (coated at 3 µg/mL) in the presence of soluble LewisA or LewisX tetrasaccharides (1.0-0.01 mM). Bound antibody was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CA19-9 antibody [GTX933] (GTX635389) diluted at 1:1000 and competitor's antibody (MA5-12421) diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9 antibody [GTX933] (GTX635389) as capture antibody at concentration of 5 µg/mL and HRP-conjugated Ca19-9 antibody [GT8510] (GTX635391) as detection antibody at concentration of 1 µg/mL. Please notice that GTX635391 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635389. Please contact us for custom HRP-conjugated antibody.
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Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9 antibody [GT8510] (GTX635391) as capture antibody at concentration of 5 µg/mL and HRP-conjugated CA19-9 antibody [GT933] (GTX635389) as detection antibody at concentration of 1 µg/mL. Please notice that GTX635389 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635391. Please contact us for custom HRP-conjugated antibody.
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CA19-9 antibody [GTX933] detects CA19-9 by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic ductal adenocarcinoma. CA19-9 stained by CA19-9 antibody [GTX933] (GTX635389) diluted at 1:500 and competitor's antibody diluted at 1:250 (Company A) and 1:500 (Company B). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
GTX635389 Image
CA19-9 antibody [GTX933] detects CA19-9 by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic ductal adenocarcinoma. CA19-9 stained by CA19-9 antibody [GTX933] (GTX635389) diluted at 1:500 and competitor's antibody diluted at 1:250 (Company A) and 1:500 (Company B). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
GTX635389 Image
CA19-9 antibody [GTX933] detects CA19-9 by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic ductal adenocarcinoma. CA19-9 stained by CA19-9 antibody [GTX933] (GTX635389) diluted at 1:500 and competitor's antibody (Company A) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
GTX635389 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CA19-9 antibody [GTX933] (GTX635389) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT933 |
| Isotype: | IgG1 |
| Immunogen: | The immunogen used to generate this antibody corresponds to CA19-9. |
| Application: | ELISA, FC, ICC, IF, IHC-P, sELISA, WB |
Additional Text
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
Carbohydrate antigen 19-9 (CA 19-9), also known as sialyl-LewisA, is a tetrasaccharide with the sequence Neu5Acα2-3Galβ1-3[Fucα1-4]GlcNAcβ. It is used clinically to assist in the diagnosis of pancreatic cancer, but is more widely utilized to monitor therapy and detect recurrences in diagnosed cases. CA 19-9 is also elevated in many other diseases, including cholangiocarcinoma, colorectal cancer, hepatocellular carcinoma, and cirrhosis. GeneTex's CA 19-9 mouse monoclonal antibody [GT933] demonstrates superior sensitivity for western blot, immunohistochemistry, and immunocytochemistry in comparative testing against other well-known CA 19-9 antibodies. Most significantly, glycan array interrogation using this antibody establishes that it is specific for sialyl-LewisA.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX635389 Datasheet
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