PAX3 antibody [HL160]
Catalogue Number: GTX635463-GTX
GTX635463 Image
GTX635463 Image
GTX635463 Image
GTX635463 Image
GTX635463 Image
PAX3 antibody [HL160] detects PAX3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E10.5 embryo. Green: PAX3 stained by PAX3 antibody [HL160] (GTX635463) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX635463 Image
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] (GTX635463) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635463 Image
U87-MG whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] (GTX635463) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635463 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] (GTX635463) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | paired box 3 , CDHS , HUP2 , WS1 , WS3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL160 |
| Isotype: | IgG |
| Immunogen: | The immunogen used to generate this antibody corresponds to Coturnix coturnix PAX3. |
| Application: | IHC-P, WB |
Description
Description: This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
PAX3
Gene ID
5077
Concentration
1.5 mg/ml
Uniprot ID
P23760
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX635463 Datasheet
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