53BP1 antibody [HL275]
Catalogue Number: GTX635479-GTX
GTX635479 Image
GTX635479 Image
GTX635479 Image
GTX635479 Image
GTX635479 Image
GTX635479 Image
GTX635479 Image
GTX635479 Image
GTX635479 Image
GTX635479 Image
GTX635479 Image
GTX635479 Image
53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 53BP1 stained by 53BP1 antibody [HL275] (GTX635479) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX635479 Image
53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 53BP1 stained by 53BP1 antibody [HL275] (GTX635479) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 µm.
GTX635479 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635479 Image
Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635479 Image
HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635479 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635479 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635479 Image
53BP1 antibody [HL275] detects 53BP1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. 53BP1 stained by 53BP1 antibody [HL275] (GTX635479) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX635479 Image
53BP1 antibody [HL275] detects 53BP1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. 53BP1 stained by 53BP1 antibody [HL275] (GTX635479) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX635479 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL275] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GTX635479 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL275] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 214 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | tumor protein p53 binding protein 1 , 53BP1 , TDRD30 , p202 , p53BP1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL275 |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human 53BP1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
Additional Text
Uniprot ID
Q12888
Gene ID
7158
Concentration
1.4 mg/mL
Purification
Protein A purified
Gene Name
TP53BP1
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX635479 Datasheet
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