SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6]

Catalogue Number: GTX635654-GTX

GTX635654 Image

SARS-CoV-2 (COVID-19) spike antibody [HL6] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis. Sample: Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).

GTX635654 Image

Indirect ELISA analysis was performed by coating plate with 50 µL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein at concentrations ranging from 0.0625 µg/mL to 4 µg/mL. The coated protein is detected with (GTX635654) at 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635654 Image

Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts (GTX535664) using GTX632604 as capture antibody at concentration of 5 µg/mL and GTX635654 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635654 Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein or recombinant SARS-CoV spike S1 subunit protein (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 µg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

GTX635654 Image

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX635654 Image

SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) spike protein by flow cytometry analysis. Sample: 293T cells transfected SARS-CoV-2 (2019-nCoV) Spike. Blue: Unlabelled sample was used as a control. Red: SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.

GTX635654 Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640). Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635654 Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 µg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635654 Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis of SARS-CoV-2 infected human lung. The IHC-P was performed by HISTOWIZ.

GTX635654 Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right). The IHC-P was performed by HISTOWIZ.

GTX635654 Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (2.4*10-⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody. EC50 : 0.251 nM

GTX635654 Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] detects SARS-CoV-2 (COVID-19) Spike S1 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:2000.

GTX635654 Image

Previous Next

Manufacturer:	GeneTex
Physical state:	Liquid
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 25 ul
Host name:	Rabbit
Clone:	HL6
Isotype:	IgG
Immunogen:	Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1). (SARS-CoV-2 (strain Wuhan-Hu-1)) The exact sequence is proprietary.
Application:	ELISA, FACS, ICC, IF, IHC-P, sELISA, WB, IHC