SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263]
Catalogue Number: GTX635708-GTX
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GTX635708 Image
GTX635708 Image
GTX635708 Image
GTX635708 Image
GTX635708 Image
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Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (501.V1) spike protein, SARS-CoV-2 (501.V2) spike protein and SARS-CoV-2 spike protein (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) (1 µg/mL). Mouse IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
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Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) as capture antibody at concentration of 5 µg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640). Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX635708 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX635708 Image
Non-transfected (-) and transfected (+) Vero E6 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | 2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT263 |
| Isotype: | IgG2a |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) Spike. (SARS-CoV-2 (strain Wuhan-Hu-1)) The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, sELISA, WB, IHC |
Additional Text
Concentration
1 mg/ml
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Gene Alias
2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike
GTX635708 Datasheet
SUPPORT
outstanding technical support
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DELIVERY
we offer free delivery to UK universities and non profit organisations