VPS35 antibody [HL1017]
Catalogue Number: GTX635821-GTX
GTX635821 Image
GTX635821 Image
GTX635821 Image
GTX635821 Image
GTX635821 Image
GTX635821 Image
GTX635821 Image
GTX635821 Image
GTX635821 Image
GTX635821 Image
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635821 Image
Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635821 Image
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635821 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635821 Image
VPS35 antibody detects VPS35 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS35 stained by VPS35 antibody (GTX635821) diluted at 1:500.
GTX635821 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635821 Image
Whole zebrafish extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX635821 Image
VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX635821 Image
VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX635821 Image
VPS35 antibody [HL1017] detects VPS35 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 92 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | VPS35 retromer complex component , MEM3 , PARK17 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1017 |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]
Additional Text
Uniprot ID
Q96QK1
Gene ID
55737
Purification
Protein A purified
Gene Name
VPS35
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX635821 Datasheet
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