PD-L1 antibody [HL1041]
Catalogue Number: GTX635975-GTX
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000 and competitor's antibody diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis. Sample: Human Placenta. Green: PD-L1 stained by Dylight594-conjugated PD-L1 antibody [HL1041] (GTX635975) diluted at 1:1000.
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PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:1000 and competitor's antibody diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
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Wild-type (WT) and PDL1 knockout (KO) MDA-MB-231 cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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PD-L1 antibody [HL1041] detects PD-L1 protein by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in ice-cold MeOH for 10 min. Green: PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
GTX635975 Image
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX635975 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GTX635975 Image
PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded A549 (left) and MDA-MB-231 xenograft (right). PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus (left) and esophageal adenocarcinoma (right). PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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PD-L1 antibody [HL1041] (GTX635975) detects PD-L1 protein by intracellular flow cytometry analysis. Sample: MDA-MB-231 or A549 cells were fixed in 4% paraformaldehyde at 4oC for 15 min and washed with permeabilization buffer. The cells were following stained with 5µg/ml GTX635975 (red) or a Rabbit monoclonal IgG isotype control (gray) at 4oC for 1 hour.
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PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lymph node. PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human thymus gland. PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX635975 Image
PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Molecular Weight: | 33 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1041 |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide encompassing a sequence within the Intracellular domain of human PD-L1. The exact sequence is proprietary. |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Additional Text
Uniprot ID
Q9NZQ7
Gene ID
29126
Purification
Protein A purified
Gene Name
CD274
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX635975 Datasheet
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