SQSTM1 / P62 antibody [HL1113]
Catalogue Number: GTX636328-GTX
GTX636328 Image
GTX636328 Image
GTX636328 Image
GTX636328 Image
GTX636328 Image
GTX636328 Image
GTX636328 Image
GTX636328 Image
GTX636328 Image
SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at autophagosome by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX636328 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636328 Image
Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636328 Image
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636328 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
GTX636328 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636328 Image
Untreated (-) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 48 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1113 |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
Additional Text
Gene Name
SQSTM1
Uniprot ID
Q13501
Gene ID
8878
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX636328 Datasheet
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