SDHA antibody [HL1117]
Catalogue Number: GTX636335-GTX
GTX636335 Image
GTX636335 Image
GTX636335 Image
GTX636335 Image
GTX636335 Image
GTX636335 Image
GTX636335 Image
GTX636335 Image
GTX636335 Image
GTX636335 Image
GTX636335 Image
GTX636335 Image
GTX636335 Image
GTX636335 Image
Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636335 Image
Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636335 Image
SDHA antibody [HL1117] detects SDHA protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636335 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636335 Image
Whole zebrafish extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636335 Image
SDHA antibody [HL1117] detects SDHA protein by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636335 Image
SDHA antibody [HL1117] detects SDHA protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636335 Image
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636335 Image
SDHA antibody [HL1117] detects SDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX636335 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636335 Image
HepG2 and mitochondria extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636335 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636335 Image
SDHA antibody [HL1117] detects SDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:500. Red: MitoTrackerR Red CMXRos, a mitochondria marker. Blue: Fluoroshield with DAPI (GTX30920).
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1117 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-WM |
Description
Description: This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
Additional Text
Gene Name
SDHA
Uniprot ID
P31040
Gene ID
6389
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX636335 Datasheet
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