Thrombospondin 2 antibody [HL1140]
Catalogue Number: GTX636411-GTX
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AsPC-1 whole cell extract and conditioned medium (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (GTX636411) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (GTX636411) diluted at 1:300000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (GTX636411) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1140] (GTX636411) (5 µg/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1194] (GTX636506) (1 µg/mL) Please notice that GTX636506 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636411. Please contact us for custom HRP-conjugated antibody.
GTX636411 Image
Sandwich ELISA detection of recombinant human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1194] (GTX636506) (5 µg/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1140] (GTX636411) (1 µg/mL) Please notice that GTX636411 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636506. Please contact us for custom HRP-conjugated antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 130 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | thrombospondin 2 , TSP2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 25 ul, 100 ul |
| Host name: | Rabbit |
| Clone: | HL1140 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Thrombospondin 2. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, sELISA, WB |
Description
Description: The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008]
Additional Text
Uniprot ID
P35442
Gene Name
THBS2
Gene ID
7058
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX636411 Datasheet
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