AKT1 antibody [HL1142]
Catalogue Number: GTX636413-GTX
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GTX636413 Image
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AKT1 antibody [HL1142] detects AKT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636413 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 | 10 | 5 | 50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Wild-type (WT) and AKT1 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636413 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636413 Image
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636413 Image
AKT1 antibody [HL1142] detects AKT1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat mammary gland. AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636413 Image
AKT1 antibody [HL1142] detects AKT1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog mammary gland. AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636413 Image
AKT1 antibody [HL1142] detects AKT1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1142 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human AKT1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Additional Text
Gene Name
AKT1
Gene ID
207
Uniprot ID
P31749
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX636413 Datasheet
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