Integrin beta 1 / CD29 antibody [HL1255]
Catalogue Number: GTX636657-GTX
GTX636657 Image
GTX636657 Image
GTX636657 Image
GTX636657 Image
GTX636657 Image
GTX636657 Image
GTX636657 Image
GTX636657 Image
GTX636657 Image
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636657 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
GTX636657 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GTX636657 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (100 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX636657 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636657 Image
Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein by immunohistochemical analysis. Sample: Paraffin-embedded dog tissues. Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636657 Image
Wild-type (WT) and ITGB1 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636657 Image
Immunoprecipitation of Integrin beta 1 / CD29 protein from A431 whole cell extract using 5 µg of Integrin beta 1 / CD29 antibody [HL1255] (GTX636657). Western blot analysis was performed using Integrin beta 1 / CD29 antibody [HL1255] (GTX636657). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).
GTX636657 Image
Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 88 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1255 |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide encompassing a sequence within the Intracellular domain of human Integrin beta 1 / CD29. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Additional Text
Gene ID
3688
Gene Name
ITGB1
Uniprot ID
P05556
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:5000-1:20000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
Integrin beta-1 / CD29 antibody detects integrin beta-1 protein (ITGB1), also known as CD29, which forms heterodimeric receptors with at least ten integrin alpha proteins to mediate various aspects of cell adhesion and migration. These functions are essential for embryogenesis, tissue repair, immune function, and other crucial processes in multicellular organisms. Integrin beta-1 has a predicted molecular weight of 88 kDa and exists in multiple isoforms.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX636657 Datasheet
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