Carbonic Anhydrase IX antibody [HL1410]
Catalogue Number: GTX636876-GTX
GTX636876 Image
GTX636876 Image
GTX636876 Image
GTX636876 Image
GTX636876 Image
Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636876 Image
Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636876 Image
Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein at cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10µm.
GTX636876 Image
Untreated (-) and treated (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 50 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | carbonic anhydrase 9 , CAIX , MN |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1410 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the Extracellular domain of human Carbonic Anhydrase IX. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
Additional Text
Gene ID
768
Gene Name
CA9
Uniprot ID
Q16790
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX636876 Datasheet
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