IL1 beta antibody [HL1423]
Catalogue Number: GTX636889-GTX
GTX636889 Image
GTX636889 Image
GTX636889 Image
GTX636889 Image
GTX636889 Image
GTX636889 Image
Sandwich ELISA detection of recombinant IL1 beta using antibodies as below. Capture: IL1 beta antibody [HL1421] (GTX636887) (5 µg/mL) Detection: HRP-conjugated IL1 beta antibody [HL1423] (GTX636889) (1 µg/mL) Please notice that GTX636889 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636887. Please contact us for custom HRP-conjugated antibody.
GTX636889 Image
Sandwich ELISA detection of recombinant IL1 beta using antibodies as below. Capture: IL1 beta antibody [HL1423] (GTX636889) (5 µg/mL) Detection: HRP-conjugated IL1 beta antibody [HL1421] (GTX636887) (1 µg/mL) Please notice that GTX636887 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636889. Please contact us for custom HRP-conjugated antibody.
GTX636889 Image
IL1 beta antibody [HL1423] detects secreted IL1 beta protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. IL1 beta stained by IL1 beta antibody [HL1423] (GTX636889) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636889 Image
IL1 beta antibody [HL1423] detects IL1 beta protein at cytoplasm by immunofluorescent analysis. Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL1 beta stained by IL1 beta antibody [HL1423] (GTX636889) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
GTX636889 Image
Untreated (-) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [HL1423] (GTX636889) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 31 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | interleukin 1 beta , IL-1 , IL1-BETA , IL1F2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1423 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human IL1 beta. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, sELISA, WB |
Description
Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
IL1B
Gene ID
3553
Uniprot ID
P01584
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX636889 Datasheet
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