Dopamine Receptor D2 antibody [HL1478]
Catalogue Number: GTX636952-GTX
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Unboiled SH-SY5Y whole cell and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
GTX636952 Image
Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636952 Image
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in ice-cold MeOH for 5 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10µm.
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Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000 and competitor's antibody (Ab5084p) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
GTX636952 Image
Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Dopamine Receptor D2 antibody [HL1478] (GTX636952) specifically detects DRD2 protein on a VirDTM-GPCR array. Yellow: DRD2 White: DRD3 Blue: DRD5 Bottom left: Positive control
GTX636952 Image
Unboiled mouse tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | dopamine receptor D2 , D2DR , D2R |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1478 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the Extracellular domain human of Dopamine Receptor D2. The exact sequence is proprietary. |
| Application: | ICC, IF, WB, FA |
Description
Description: This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
DRD2
Uniprot ID
P14416
Gene ID
1813
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX636952 Datasheet
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