Vimentin antibody [HL1506]
Catalogue Number: GTX636980-GTX
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Vimentin antibody [HL1506] detects Vimentin protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Wild-type (WT) and Vimentin knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636980 Image
Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636980 Image
Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissue. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636980 Image
Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat lymph node. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636980 Image
Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Vimentin antibody [HL1506] detects Vimentin protein at cytoskeleton and cell junction by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
GTX636980 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | vimentin |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1506 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Additional Text
Gene Name
VIM
Gene ID
7431
Uniprot ID
P08670
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX636980 Datasheet
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