EpCAM antibody [HL1524]
Catalogue Number: GTX636998-GTX
GTX636998 Image
GTX636998 Image
GTX636998 Image
GTX636998 Image
GTX636998 Image
GTX636998 Image
GTX636998 Image
GTX636998 Image
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with EpCAM antibody [HL1524] (GTX636998) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636998 Image
EpCAM antibody [HL1524] detects EpCAM protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636998 Image
EpCAM antibody [HL1524] detects EpCAM protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636998 Image
EpCAM antibody [HL1524] (GTX636998) detects EpCAM protein by flow cytometry analysis. Sample: HCT-116, HeLa and 4T1 cell. Gray: Rabbit IgG Isotype control. Red: EpCAM antibody [HL1524] (GTX636998) dilution: 1:50. Acquisition of 50,000 (HCT-116 and HeLa cell) and 100,000 (4T1 cell) events were collected for flow cytometry analysis.
GTX636998 Image
EpCAM antibody [HL1524] detects EpCAM protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636998 Image
EpCAM antibody [HL1524] detects EpCAM protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded HCT116 xenograft. EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX636998 Image
EpCAM antibody [HL1524] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis. Sample: HCT116 and HeLa cells were fixed in ice-cold MeOH for 5 min. Green: EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 35 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1524 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the Extracellular domain of human EpCAM. The exact sequence is proprietary. |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Additional Text
Gene ID
4072
Gene Name
EPCAM
Uniprot ID
P16422
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX636998 Datasheet
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