B-Raf antibody [HL1606]
Catalogue Number: GTX637085-GTX
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GTX637085 Image
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GTX637085 Image
GTX637085 Image
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1606] (GTX637085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX637085 Image
Wild-type (WT) and BRAF knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1606] (GTX637085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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B-Raf antibody [HL1606] detects B-Raf protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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B-Raf antibody [HL1606] detects B-Raf protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX637085 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1606] (GTX637085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX637085 Image
B-Raf antibody [HL1606] detects B-Raf protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX637085 Image
B-Raf antibody [HL1606] detects B-Raf protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX637085 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1606] (GTX637085) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX637085 Image
B-Raf antibody [HL1606] detects B-Raf protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 84 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1606 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human B-Raf. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
Additional Text
Gene Name
BRAF
Uniprot ID
P15056
Gene ID
673
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX637085 Datasheet
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