beta Catenin antibody [HL1753]
Catalogue Number: GTX637403-GTX
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GTX637403 Image
GTX637403 Image
GTX637403 Image
GTX637403 Image
GTX637403 Image
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [HL1753] (GTX637403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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beta Catenin antibody [HL1753] detects beta Catenin protein at cell membrane and cell junction by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin stained by beta Catenin antibody [HL1753] (GTX637403) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX637403 Image
beta Catenin antibody [HL1753] detects beta Catenin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded cat mammary gland. beta Catenin stained by beta Catenin antibody [HL1753] (GTX637403) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX637403 Image
beta Catenin antibody [HL1753] detects beta Catenin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded dog mammary gland. beta Catenin stained by beta Catenin antibody [HL1753] (GTX637403) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX637403 Image
Wild-type (WT) and CTNNB1 knockout (KO) 293T cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [HL1753] (GTX637403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX637403 Image
beta Catenin antibody [HL1753] detects beta Catenin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. beta Catenin stained by beta Catenin antibody [HL1753] (GTX637403) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 85 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1753 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human beta Catenin |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Additional Text
Gene Name
CTNNB1
Gene ID
1499
Uniprot ID
P35222
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX637403 Datasheet
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