EPO antibody [HL1794]
Catalogue Number: GTX637446-GTX
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Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (GTX00422-pro) (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (GTX637446) (1 µg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
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Escherichia coli expressed recombinant human EPO protein were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (GTX637446) (1 µg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
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Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1793] (GTX637445) (5 µg/mL) Detection: HRP-conjugated EPO antibody [HL1794] (GTX637446) (1 µg/mL) Please notice that GTX637446 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637445. Please contact us for custom HRP-conjugated antibody.
GTX637446 Image
Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1794] (GTX637446) (5 µg/mL) Detection: HRP-conjugated EPO antibody [HL1793] (GTX637445) (1 µg/mL) Please notice that GTX637445 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637446. Please contact us for custom HRP-conjugated antibody.
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Recombinant human EPO protein (CHO cells expressed) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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HepG2 whole cell extract and conditioned medium (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. EPO stained by EPO antibody [HL1794] (GTX637446) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. EPO stained by EPO antibody [HL1794] (GTX637446) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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The cell proliferation of the TF-1 human erythroleukemic cell line induced by Human Erythropoietin/EPO and neutralization by Erythropoietin Antibody. Proliferation elicited by Recombinant Human Erythropoietin/EPO (0.5 ng/mL) is neutralized by increasing concentrations of EPO antibody [HL1794] (GTX637446).
GTX637446 Image
Sandwich ELISA detection of recombinant (CHO cells expressed, full-length human EPO protein) using antibodies as below. Capture: EPO antibody [HL1793] (GTX637445) (5 µg/mL) Detection: HRP-conjugated EPO antibody [HL1794]? (GTX637446-01) (1 µg/mL
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 21 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | erythropoietin , DBAL , ECYT5 , EP , MVCD2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1794 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human EPO |
| Application: | ELISA, IHC-P, sELISA, WB, NT, Inh |
Description
Description: This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]
Additional Text
Gene Name
EPO
Gene ID
2056
Uniprot ID
P01588
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Molecular Weight
21
Concentration
1 mg/ml
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX637446 Datasheet
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