GPRC5A antibody [HL1864]
Catalogue Number: GTX637589-GTX
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GPRC5A antibody [HL1864] detects GPRC5A protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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Boiled and unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A? antibody [HL1864] (GTX637589) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Non-transfected (-) and transfected (+) unboiled MCF-7 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A? antibody [HL1864] (GTX637589) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A? antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A? antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL1864] (GTX637589) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GPRC5A antibody [HL1864] detects GPRC5A protein by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GPRC5A antibody [HL1864] detects GPRC5A protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GPRC5A antibody [HL1864] detects GPRC5A protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GPRC5A antibody [HL1864] detects GPRC5A protein by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:100 and highly cited competitor's antibody diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
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GPRC5A antibody [HL1864] detects GPRC5A protein by immunofluorescent analysis. Green: GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920)
GTX637589 Image
Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX637589 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | G protein-coupled receptor class C group 5 member A , GPCR5A , PEIG-1 , RAI3 , RAIG1 , TIG1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1864 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the Intracellular domain of human RAI3. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a member of the type 3 G protein-coupling receptor family, characterized by the signature 7-transmembrane domain motif. The encoded protein may be involved in interaction between retinoid acid and G protein signalling pathways. Retinoic acid plays a critical role in development, cellular growth, and differentiation. This gene may play a role in embryonic development and epithelial cell differentiation. [provided by RefSeq, Jul 2008]
Additional Text
Gene ID
9052
Gene Name
GPRC5A
Uniprot ID
Q8NFJ5
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX637589 Datasheet
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