Ataxin 3 antibody [HL1909]
Catalogue Number: GTX637658-GTX
GTX637658 Image
GTX637658 Image
GTX637658 Image
GTX637658 Image
GTX637658 Image
GTX637658 Image
GTX637658 Image
GTX637658 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Ataxin 3 antibody [HL1909] detects Ataxin 3 protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10µm.
GTX637658 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX637658 Image
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX637658 Image
Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX637658 Image
Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX637658 Image
Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | ataxin 3 , AT3 , ATX3 , JOS , MJD , MJD1 , SCA3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1909 |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment of human Ataxin 3. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 12-44 to 52-86 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2016]
Additional Text
Gene Name
ATXN3
Uniprot ID
P54252
Gene ID
4287
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX637658 Datasheet
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