CD97 antibody [HL1925]
Catalogue Number: GTX637674-GTX
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GTX637674 Image
GTX637674 Image
GTX637674 Image
GTX637674 Image
GTX637674 Image
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Immunoprecipitation of CD97 protein from U937 using 5 µg of CD97 antibody [HL1925] (GTX637674). Western blot analysis was performed using CD97 antibody [HL1925] (GTX637674). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).
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Boiled and unboiled U937 whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Untreated (-) and treated (+) U937 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GTX637674 Image
Wild-type (WT) and ADGRE5 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX637674 Image
U937 whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:1000 and competitor's antibody (Clone EPR4427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.
GTX637674 Image
Wild-type (WT) and CD97 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:1000 and the highly cited competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | adhesion G protein-coupled receptor E5 , CD97 , TM7LN1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1925 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the Extracellular domain of human CD97. The exact sequence is proprietary. |
| Application: | IP, WB |
Description
Description: This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]
Additional Text
Uniprot ID
P48960
Concentration
2 mg/ml
Gene ID
976
Purification
Protein A purified
Gene Name
ADGRE5
Antibody Clonality
Recombinant Monoclonal
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX637674 Datasheet
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