IGF2 antibody [HL1982]
Catalogue Number: GTX637875-GTX
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GTX637875 Image
GTX637875 Image
GTX637875 Image
GTX637875 Image
GTX637875 Image
GTX637875 Image
GTX637875 Image
HepG2 whole cell extract and conditioned medium (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (GTX637875) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (GTX637875) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, mature recombinant human IGF2 protein (533.33-8.33 nM). Coated protein was probed with IGF2 antibody [HL1982] (GTX637875) (1 µg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
GTX637875 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (GTX637875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX637875 Image
Mouse plasma (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (GTX637875) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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IGF2 antibody [HL1982] detects IGF2 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2 stained by IGF2 antibody [HL1982] (GTX637875) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX637875 Image
Sandwich ELISA detection of recombinant E.coli expressed, mature recombinant human IGF2 protein using antibodies as below. Capture: IGF2 antibody [HL2937] (GTX640321) (5 µg/mL) Detection: HRP-conjugated IGF2 antibody [HL1982] (GTX637875) (1 µg/mL) Please notice that GTX637875 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640321. Please contact us for custom HRP-conjugated antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 20 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | insulin like growth factor 2 , C11orf43 , GRDF , IGF-II , PP9974 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1982 |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment of human IGF2 |
| Application: | ELISA, ICC, IF, sELISA, WB |
Description
Description: This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
Additional Text
Gene Name
IGF2
Gene ID
3481
Uniprot ID
P01344
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX637875 Datasheet
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