RPL32 antibody [HL2331]
Catalogue Number: GTX638494-GTX
GTX638494 Image
GTX638494 Image
GTX638494 Image
GTX638494 Image
GTX638494 Image
GTX638494 Image
GTX638494 Image
GTX638494 Image
GTX638494 Image
GTX638494 Image
GTX638494 Image
GTX638494 Image
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (GTX638494) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX638494 Image
Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (GTX638494) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX638494 Image
Whole zebrafish extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (GTX638494) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX638494 Image
RPL32 antibody [HL2331] detects RPL32 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. RPL32 stained by RPL32 antibody [HL2331] (GTX638494) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX638494 Image
RPL32 antibody [HL2331] detects RPL32 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. RPL32 stained by RPL32 antibody [HL2331] (GTX638494) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX638494 Image
Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (GTX638494) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX638494 Image
RPL32 antibody [HL2331] detects RPL32 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL32 stained by RPL32 antibody [HL2331] (GTX638494) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX638494 Image
RPL32 antibody [HL2331] detects RPL32 protein by immunohistochemical analysis. Sample: Paraffin-embedded dog tissues. RPL32 stained by RPL32 antibody [HL2331] (GTX638494) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX638494 Image
RPL32 antibody [HL2331] detects RPL32 protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: RPL32 stained by RPL32 antibody [HL2331] (GTX638494) diluted at 1:100. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70oC
GTX638494 Image
RPL32 antibody [HL2331] detects RPL32 protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: RPL32 stained by RPL32 antibody [HL2331] (GTX638494) diluted at 1:100. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70oC
GTX638494 Image
RPL32 antibody [HL2331] detects RPL32 protein by immunofluorescent analysis. Sample: MDCK cells were fixed in ice-cold MeOH for 5 min. Green: RPL32 stained by RPL32 antibody [HL2331] (GTX638494) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Molecular Weight: | 16 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | ribosomal protein L32 , L32 , PP9932 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL2331 |
| Isotype: | IgG |
| Immunogen: | Full length of human RPL32 recombinant protein. |
| Application: | ICC, IF, IHC-P, WB, IHC-WM |
Description
Description: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
RPL32
Gene ID
6161
Uniprot ID
P62910
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX638494 Datasheet
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