IL6 antibody [HL2389]
Catalogue Number: GTX638598-GTX
GTX638598 Image
GTX638598 Image
GTX638598 Image
GTX638598 Image
GTX638598 Image
GTX638598 Image
GTX638598 Image
GTX638598 Image
GTX638598 Image
IL6 antibody [HL2389] detects IL6 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. IL6 stained by IL6 antibody [HL2389] (GTX638598) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX638598 Image
IL6 antibody [HL2389] detects IL6 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. IL6 stained by IL6 antibody [HL2389] (GTX638598) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX638598 Image
Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (2634.91-41.32 pM). Coated protein was probed with IL6 antibody [HL2389] (GTX638598) (1 µg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
GTX638598 Image
Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT1535] (GTX636571) (5 µg/mL) Detection: IL6 antibody [HL2389] (GTX638598) (1 µg/mL)
GTX638598 Image
Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT519-1] (GTX636573) (5 µg/mL) Detection: IL6 antibody [HL2389] (GTX638598) (1 µg/mL)
GTX638598 Image
Recombinant IL6 were separated by 15% SDS-PAGE, and the membranes were blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
GTX638598 Image
Untreated (-) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX638598 Image
Untreated (-) and treated (+) THP-1 whole cell extract (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 24 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL2389 |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment of human IL6. |
| Application: | ELISA, IHC-P, sELISA, WB |
Description
Description: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Additional Text
Gene Name
IL6
Gene ID
3569
Uniprot ID
P05231
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX638598 Datasheet
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