Adenosine A1 Receptor antibody [HL2442]
Catalogue Number: GTX638758-GTX
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Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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SH-SY5Y whole cell and membrane extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 µg) were separated by 15% SDS-PAGE, and the membranes were blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:2000 and the highly cited competitor's diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX638758 Image
Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX638758 Image
Non-transfected (-) and transfected (+) MCF-7 whole cell extract (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX638758 Image
Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | No preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | adenosine A1 receptor , RDC7 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL2442 |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide encompassing a sequence within the Intracellular domain of human Adenosine A1 Receptor. The exact sequence is proprietary. |
| Application: | IHC-P, WB |
Description
Description: The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
ADORA1
Gene ID
134
Uniprot ID
P30542
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX638758 Datasheet
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