Tau (phospho Thr181) antibody [HL2497]
Catalogue Number: GTX638855-GTX
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Untreated (-) and treated (+) SK-N-SH whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2497] (GTX638855) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Whole cell and rat tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2497] (GTX638855) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX638855 Image
Tau (phospho Thr181) antibody [HL2497] detects Tau (phospho Thr181) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right). Sample: Paraffin-embedded mouse brain. Tau (phospho Thr181) stained by Tau (phospho Thr181) antibody [HL2497] (GTX638855) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX638855 Image
Untreated (-) and treated (+) mouse tissue extract (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2497] (GTX638855) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Sandwich ELISA detection of Tau peptide and Tau (phospho Thr181) peptide using antibodies as below. Capture: Tau (phospho Thr181) antibody [HL2498] (GTX638856) (5 µg/mL) Detection: HRP-conjugated Tau (phospho Thr181) antibody [HL2497] (GTX638855) (1 µg/mL) Please notice that GTX638855 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638856. Please contact us for custom HRP-conjugated antibody.
GTX638855 Image
Sandwich ELISA detection of Tau peptide and Tau (phospho Thr181) peptide using antibodies as below. Capture: Tau (phospho Thr181) antibody [HL2497] (GTX638855) (5 µg/mL) Detection: HRP-conjugated Tau (phospho Thr181) antibody [HL2498] (GTX638856) (1 µg/mL) Please notice that GTX638856 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638855. Please contact us for custom HRP-conjugated antibody.
| Manufacturer: | GeneTex |
| Preservative: | No preservative |
| Molecular Weight: | 79 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL2497 |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide surrounding phospho Thr181 of human Tau. The exact sequence is proprietary. |
| Application: | ELISA, IHC-P, sELISA, WB |
Description
Description: This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
MAPT
Gene ID
4137
Uniprot ID
P10636
Modification
Phosphorylated, Thr181
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. IHC-P: 1:44-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX638855 Datasheet
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