AGTR1 antibody [HL2524]
Catalogue Number: GTX638885-GTX
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GTX638885 Image
GTX638885 Image
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GTX638885 Image
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AGTR1 antibody [HL2524] (GTX638885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GTX638885 Image
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with AGTR1 antibody [HL2524] (GTX638885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX638885 Image
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with AGTR1 antibody [HL2524] (GTX638885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
GTX638885 Image
AGTR1 antibody [HL2524] detects AGTR1 protein at cell membrane by immunofluorescent analysis. Sample: Huh-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AGTR1 stained by AGTR1 antibody [HL2524] (GTX638885) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
GTX638885 Image
AGTR1 antibody [HL2524] detects AGTR1 protein by immunofluorescent analysis. Sample: Huh-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AGTR1 stained by AGTR1 antibody [HL2524] (GTX638885) diluted at 1:500 and competitor's antibody (competitor) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
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AGTR1 antibody [HL2524] detects AGTR1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded Huh-7 xenograft. AGTR1 stained by AGTR1 antibody [HL2524] (GTX638885) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX638885 Image
Huh-7 cytoplasma extracts and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AGTR1 antibody [HL2524] (GTX638885) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (CE: cytoplasma extract; ME: membrane extract)
GTX638885 Image
AGTR1 antibody [HL2524] detects AGTR1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. AGTR1 stained by AGTR1 antibody [HL2524] (GTX638885) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | No preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | angiotensin II receptor type 1 , AG2S , AGTR1B , AT1 , AT1AR , AT1B , AT1BR , AT1R , AT2R1 , HAT1R |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL2524 |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide encompassing a sequence within the Extracellular domain of human AGTR1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012]
Additional Text
Gene ID
185
Uniprot ID
P30556
Gene Name
AGTR1
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX638885 Datasheet
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