EGFRvIII antibody [HL2541]
Catalogue Number: GTX638910-GTX
GTX638910 Image
GTX638910 Image
GTX638910 Image
GTX638910 Image
GTX638910 Image
GTX638910 Image
GTX638910 Image
GTX638910 Image
EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
GTX638910 Image
EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
GTX638910 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX638910 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
GTX638910 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
GTX638910 Image
EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX638910 Image
EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunohistochemical analysis. Sample: EGFRvIII transfected 293T cell FFPE Cell Pellet Block. Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | No preservative |
| Molecular Weight: | 134 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | EGFRvIII,EGFR mutant,EGFR variant,EGFRv3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL2541 |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide of human EGFRvIII. |
| Application: | ICC, IF, WB, IHC |
Description
Description: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Additional Text
Gene ID
1956
Gene Name
EGFR
Uniprot ID
P00533
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX638910 Datasheet
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