Smoothened homolog antibody [HL2975]
Catalogue Number: GTX640362-GTX
GTX640362 Image
GTX640362 Image
GTX640362 Image
GTX640362 Image
GTX640362 Image
GTX640362 Image
Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
GTX640362 Image
Smoothened homolog antibody [HL2975] (GTX640362) specifically detects Smoothened homolog protein on a VirD?-GPCR array (CDI Labs, Baltimore, MD). Array was probed with antibody using a 1:2000 dilution. Yellow: SMO
GTX640362 Image
Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX640362 Image
Boiled and unboiled mouse brain extract (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
GTX640362 Image
Boiled and unboiled wild-type (WT) and Smoothened homolog knockout (KO) T-47D cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
| Manufacturer: | GeneTex |
| Preservative: | No preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | smoothened, frizzled class receptor , CRJS , FZD11 , Gx , SMOH |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL2975 |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment encompassing a sequence within the Intracellular domain of human Smoothened homolog. The exact sequence is proprietary. |
| Application: | WB, FA |
Description
Description: The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]
Additional Text
Gene ID
6608
Gene Name
SMO
Uniprot ID
Q99835
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX640362 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations